Effects of down-regulation of PI3K/Akt signaling pathway on growth of laryngeal cancer cells
10.3969/j.issn.1671-8348.2017.21.004
- VernacularTitle:下调PI3K/Akt信号通路对抑制喉癌细胞生长的影响
- Author:
Huimin WANG
;
Wenfa YU
;
Hang ZHOU
;
Zhenmin LU
- Keywords:
potassium channel blockers;
laryngeal neoplasms;
epithelial cells;
PI3K/Akt;
apoptosis
- From:
Chongqing Medicine
2017;46(21):2892-2894
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of potassium channel blocker tetraethtylamine (TEA) on the proliferation and apoptosis of human laryngeal carcinoma cells (Hep-2) through the PI3K/Akt signaling pathway.Methods TEA acted on HEp-2.The methyl thiazolyl blue (MTT) method was used to determine the Hep-2 cell activity and the proliferation inhibition rate of TEA on Hep-2 was calculated;the Hoechest33258 staining was used to detect cell apoptosis,and the Hep-2 apoptosis rate was detected by flow cytometry (FCM) assay.Results After inoculation by 5,10,20 mmol/L TEA,the Hep-2 cell proliferation inhibition rate reached 12.573%,31.385% and 56.132%,respectively.After 96 h Hep-2 cell action,the cellular apoptosis rates were (41.64±2.67)%,(58.76±4.32)% and (72.65±6.54)% respectively,the inhibition rate and apoptosis rate in the TEA treating group were significantly higher than those in the non-TEA treating group,the differences were statistically significant (P<0.05).Conclusion TEA can inhibit the proliferation and in vivo growth of human laryngeal carcinoma cells and promotes the apoptosis of laryngeal cancer cells.
