Effects of PRDX1 gene silencing on invasion and migration of human colorectal cancer SW480 cells
10.3969/j.issn.1000-484X.2017.07.019
- VernacularTitle:基因沉默PRDX1表达对人结直肠癌SW480细胞侵袭迁移的影响
- Author:
Jihong FENG
;
Hong ZHANG
;
Longmei LI
;
Junmin LUO
;
Chunbao ZANG
;
Hang ZHOU
- Keywords:
Peroxiredoxin 1(PRDX1);
Colorectal cancer(CRC);
Invasion and migration;
Matrix metalloproteinase(MMP);
SW480
- From:
Chinese Journal of Immunology
2017;33(7):1048-1052
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of RNA interference(RNAi)-mediated silencing of Peroxiredoxin 1(PRDX1)gene on the invasion and migration of human colorectal cancer SW480 cells.Methods: Lentiviruses negative control vector and PRDX1 RNAi were transfected respectively into colorectal cancer SW480 cells.The transfected cells were divided into PRDX1 silencing group(si-PRDX1)and negative control group(Vector).The expressions of PRDX1 mRNA and protein in SW480 cells were exa mined by quantitative real-time PCR(qRT-PCR)and immunoblotting(Western blot),respectively.The cell migration and invasion capabilities were evaluated with transwell chamber assay and transwell chamber,respectively.The protein expressions of TIMP-2,MMP-2 and MMP-9 were detected by Western blot.Results: Compared with control group,the expressions of PRDX1 mRNA and protein were significantly decreased in PRDX1 silencing group(P<0.01),PRDX1 gene silencing cell line was successfully constructed.The levels of invasion and migration capacities of SW480 cells transfected with si-PRDX1 were lower than those in the cells transfected with control-siRNA(vector)(P<0.01).The expression of TIMP-2 was significantly increased,while the expressions of MMP-2 and MMP-9 were significantly decreased(P<0.05).Conclusion: Silencing of PRDX1 inhibits the invasion,migration and metastasis of human colorectal cancer SW480 cells by regulating the expressions of TIMP-2,MMP-2 and MMP-9.
