The inhibitory effects of aloe vera extract on lipopolysaccharide-induced inflammatory response in human periodontal ligament cells
10.3969/j.issn.1001-3733.2017.04.020
- VernacularTitle:芦荟提取物对脂多糖诱导的人牙周膜细胞炎性反应的抑制作用
- Author:
Shuang ZHANG
;
Ying GAO
;
Lixian LI
;
Junrong DU
- Keywords:
Aloe vera extract(AVE);
Human periodontal ligament cells(hPDLCs);
Lipopolysaccharide(LPS)
- From:
Journal of Practical Stomatology
2017;33(4):522-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of aloe vera extra (AVE) on lipopolysaccharide (LPS)-induced inflammatory response of human periodontal ligament cells(hPDLCs).Methods:hPDLCs were induced with LPS at 1 μg/ml for the simulation of periodontitis model(model group) and then treated by AVE at 0.05,0.1,0.2 mg/ml respectively(AVE group).Cell viability was examined by MTT assay.The level of interleukin-6(IL-6) from cell culture medium was measured by ELISA.The expression of Toll like receptor 4(TLR4) protein was detected by immunocytochemistry staining and the transfer of nuclear factor-kappa B p65 (NF-κB-p65) was observed by immunofluorescence staining.Results:There was no significant difference of the cell viabilities among the groups.IL-6 in culture medium,the expression of TLR4 protein and the transfer of NF-κB-p65 into the nucleus were increased in model group.AVE at 0.05-0.2 mg/ml inhibited the secretion of IL-6 in the cell culture supernatant down-regulated the TLR4 expression,attenuated the transfer of NF-κB-p65 into the nucleus in a concentration-dependent manner.Conclusion:Aloe vera extract can inhibit the inflammation response of hPDLCs induced by LPS through TLR4/ NF-κB-p65 signaling pathway.
