Effect of Jinwu Jiangu Decoction on IL-17,ACT1,TRAF6 in Synovial Cells of Rheumatoid Arthritis
10.3870/j.issn.1004-0781.2017.06.007
- VernacularTitle:金乌健骨方对类风湿关节炎滑膜细胞IL-17和ACT1及TRAF6表达的影响
- Author:
Wukai MA
;
Qiaoyi NING
;
Dongxiao LI
;
Xueming YAO
;
Lei HOU
;
Jiang LIANG
;
Ying HUANG
;
Jing ZHOU
- Keywords:
Jinwu Jiangu decoction;
Rheumatoid arthritis;
Synovial cells;
Interleukin-17
- From:
Herald of Medicine
2017;36(6):617-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Miao medicine Jinwu Jiangu decoction containing serum freeze-dried powder on levels of IL-17,ACT1 and TRAF6 in human rheumatoid arthritis fibroblast like synoviocytes (RA-HFLS).Methods Rabbits were randomly divided into blank control group (recieving normal saline of the same volume),Jinwu Jiangu decoction high-dose,medium-dose and low-dose group (intragastrically administrated with Jinwu Jiangu decoction at doses of 14.4,4.8 and 2.4 g·kg-1,respectively),tripterygium glycosides group and prednisone group (treated with human equivalent dosage).RA-HFLS primary cell model was established in the experiment.ELISA method was used to detect effect of lyophilized powder on IL-17 secretion.Expression of ACT1,TRAF6 mRNA was detected by RT-PCR.Results Compared with the blank control gorup,IL-17 in the supernatant of each medication administration group was significantly decreased (all P<0.01),and it was decreased most significantly in Jinwu Jiangu decoction high-dose and medium-dose group.IL-17 was down-regulated more significantly in high-dose group than that in tripterygium glycosides group (P<0.01).Compared with the blank control group,TRAF6 and ACT1 mRNA expression level of each medication administration group were significantly decreased (all P<0.01),and in the high-dose group that were decreased most significantly,but not significantly different as compared with tripterygium glycosides group and prednisone group (P>0.05).Conclusion Freeze-dried powder of Jinwu Jiangu decoction can decrease the secretion of IL-17 and down-regulate expression of ACT1,TRAF6 with RA-HFLS.
