Effect of resveratrol on proliferation of liver cancer SMMC-7721 cells andlowering levels of mTOR protein phosphorylation
10.3969/j.issn.1001-1978.2017.09.023
- VernacularTitle:白藜芦醇抑制人肝癌SMMC-7721细胞增殖并降低mTOR蛋白磷酸化水平
- Author:
Tan WANG
;
Yanqun ZHANG
;
Yonglian ZENG
;
Qing XU
- Keywords:
resveratrol;
hepatic carcinoma;
cell cycle;
mTOR;
p-mTOR;
phosphory lation
- From:
Chinese Pharmacological Bulletin
2017;33(9):1309-1314
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of resveratrol on proliferation of liver cancer SMMC-7721 cells and the relevant mechanism.Methods SMMC-7721 cells were treated with different concentrations of resveratrol.Cell proliferation was tested by cell proliferation assay and colony-forming experiment.The effect of resveratrol on cell cycle of SMMC-7721 cells was detected by flow cytometry.The activation of mTOR/p-mTOR proteins was detected by Western blot.Results Compared with control group, resveratrol inhibited SMMC-7721 cell proliferation in a dose-dependent manner(P<0.01);cell cycle detection showed that SMMC-7721 cells were arrested at G0/G1 phase by resveratrol;Westren blot assay showed that resveratrol down-regulated p-mTOR expression.Conslusion Resveratrol inhibits human SMMC-7721 cell proliferation and arrests the cell cycle at G0/G1 phase, while the inhibition mechanism may be related to activation of mTOR signaling pathway by resveratrol.
