PXD101 induces apoptosis of human prostate cancer cell line PC3 by mitochondrial signal pathway

VernacularTitle:PXD101诱导人前列腺癌细胞PC3凋亡的线粒体信号通路
Author: Mingzhu HU ; Lei LI ; Lei CAO ; Yimei ZHANG ; Haihua LUO ; Shuiwang HU ; Aihua LIU ; Yong JIANG
Keywords: Prostate cancer; PXD101; Apoptosis; Mitochondria; PC3 cells
From: Chinese Journal of Pathophysiology 2017;33(5):793-797
CountryChina
Language:Chinese
Abstract: AIM:To explore the mitochondrial pathway in the apoptosis of PC3 cells induced by PXD101 (also named as belinostat).METHODS:PC3 cells were treated with PXD101 at different doses or stimulated with PXD101 for different time.The effect of PXD101 on the viability of PC3 cells was measured by CCK-8 assay.The apoptotic rates and the mitochondrial membrane potential (MMP) were analyzed by flow cytometry.The protein levels of Bcl-2, Bax and cytochrome C (Cyt C) were determined by Western blot.The caspase-3 activity were tested by caspase-3 activity assay kit.RESULTS:The viability of the PC3 cells was inhibited by PXD101 in a dose-and time-dependent manner.Flow cytometric analysis showed that the apoptotic rates were increased in the cells treated with PXD101 (P<0.01).At the same time, PXD101 induced the decreases in MMP and Bcl-2, the release of Cyt C, and the increase in caspase-3 activity.CONCLUSION:PXD101 induces the apoptosis of human prostate cancer cell line PC3 by mitochondrial signal pathway.