Assessment of effects of 7,8-dihydroxyflavone on the striatum in normal cynomolgus monkeys with 99TCm-TRODAT-1 SPECT/CT imaging
10.3760/cma.j.issn.2095-2848.2017.04.006
- VernacularTitle:7,8-二羟基黄酮对食蟹猴纹状体作用的99Tcm-TRODAT-1 SPECT/CT显像评估
- Author:
Gaohong ZHU
;
Yulin YAN
;
Lilin CHEN
;
Xuehong WANG
;
Rui HE
- Keywords:
Corpus striatum;
Dopamine plasma membrane transport proteins;
Flavones;
Tomography,emission-computed,single-photon;
Tomography,X-ray computed;
Macaca fascicularis;
TRODAT-1
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2017;37(4):216-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effects of 7,8-dihydroxyflavone (7,8-DHF) on the striatum (ST) in normal cynomolgus monkeys using 99Tcm-TRODAT-1 imaging.Methods A total of six healthy female cynomolgus monkeys were included in this study.Three of them were fed with normal food (control group),and the other three were given oral administration of 7,8-DHF in addition to normal food (experimental group).The SPECT/CT imaging was performed at different time after 99Tcm-TRODAT-1 injection.The ROI of ST was drawn on images of 3 consecutive transverse slices that could be visualized best.The cerebellum (CB) was taken as the background reference area.The radioactivity uptake ratios of ST/CB at 1,3,4 and 5 h were calculated respectively.Paired-t test was used to analyze the data.Results ST radioactive uptake ratios showed continuing increase on the delay images.ST/CB uptake ratios of the control group at 1,3,4 and 5 h were 1.43±0.04,1.82±0.06,2.04±0.12,2.42±0.23,respectively,and those of the experimental group were 1.35±0.08,2.40±0.09,2.74±0.13 and 3.25±0.15 respectively.There was no significant difference between the two groups at 1 h (t =2.57,P＞0.05),while ST/CB uptake ratios of the experimental group at 3,4 and 5 h were significantly higher (t values:2.77,2.87 and 2.92,all P＜0.05).Conclusion 99Tcm-TRODAT-1 SPECT/CT imaging can be used to assess the DAT activation effect by 7,8-DHF on ST of cynomolgus monkeys.
