Expression level of lncRNA-ENST00000460164 in luminal A breast cancer and its effect on cell cycle
- VernacularTitle:luminal A型乳腺癌组织中长链非编码RNA ENST00000460164的表达及对细胞周期的影响
- Author:
Lin ZHOU
;
Xiuwei YU
;
Kai TAO
;
Chengcheng YANG
;
Shengchun LIU
- Keywords:
luminal A breast cancer;
lncRNA ENST00000460164;
cell cycle
- From:
Basic & Clinical Medicine
2017;37(7):1037-1041
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find the expression level and the role of ENST00000460164 in luminal A breast cancer.Methods The expression level of ENST00000460164 in breast cancer tissues was detected by RT-qPCR.pll3.7-ENST00000460164-shRNA and empty vector,pll3.7,were transfected into MCF-7 cells.Cell cycle was measured by flow cytometry.Western blot was used to detect the expression of P16INK4A and cyclinD1.Results ENST00000460164 was highly expressed in luminal A breast cancer tissues as compared to the adjacent non-cancer tissues.The knockdown of ENST00000460164 resulted in the G1 cell-cycle arrested,cyclin D1 downregulated and P16INK4A upregulated in MCF-7 cells.Conclusions ENST00000460164 is overexpressed in luminal A breast cancer.ENST00000460164 may control G1/S transition by regulating P16INK4A or cyclin D1 expression.
