Determination of Total Phenylethanoid Glycosides and Acteoside in Plantago Herba
- VernacularTitle:车前草中总苯乙醇苷及毛蕊花糖苷的含量测定
- Author:
Chuanhua FENG
;
Qi REN
;
Xiaolin TANG
;
Man YI
;
Lan XU
;
Xiaoxuan TAO
;
Lang ZHANG
;
Gang LI
- Keywords:
Plantago Herba;
Phenylethanoid glycosides;
Acteoside;
Content determination
- From:
China Pharmacist
2017;20(8):1477-1479
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine total phenylethanoid glycoside and acteoside in Plantago Herba to provide reference for evaluating the quality of medicinal materials.Methods: With acteoside as the control sample, a UV visible spectrophotometric method was used to determine total phenylethanoid glycosides in Plantago Herba.An HPLC method was applied to determine acteoside in Plantago Herba , and the conditions were as follows: an ODS2 C 18 (150 mm× 4.6 mm ,5 μm) chromatographic column was used with acetonitrile-0.1% formic acid (13∶87) as the mobile phase at a flow rate of 1.0 ml·min-1 , the detection wavelength was 332nm, the column temperature was 30℃, and the sample volume was 10 μl.Results: The reference solution and the sample solution had the maximum absorption at 332 nm, and the linear relationship was good within the range of 0.003 1-0.155 0 mg·ml-1 (r=0.999 5).The content of total benzene alcohol glycosides in 3 batches of samples was 2.73% , 2.61% and 2.84% , respectively;acteoside over the range of 0.000 6-0.155 0 mg·ml-1 (r=0.999 1) showed a good linear relationship with peak area,the sample recovery was 98.5% and the RSD was 1.6% (n =6), and the acteoside content in 3 batches of samples respectively was 0.54% , 0.51% and 0.56%.Conclusion: The method is simple, accurate and reproducible, and can be used for the determination of total phenylethanoid glycosides and acteoside in Plantago Herba.
