Frozen-thawed pronuclear embryos by in vitro fertilization of C57BL/6J mice can be used for Cas9 microinjection
10.3969.j.issn.1671-7856.2017.08.013
- VernacularTitle:C57BL/6J 小鼠冻融IVF原核胚用于Cas9显微注射探讨
- Author:
Wentao ZENG
;
Jianli ZHOU
;
Shuqin ZHAO
;
Yanan ZHANG
;
Yun MEI
- Keywords:
IVF;
Fertilized eggs;
Frozen embryos;
Cas9;
Microinjection
- From:
Chinese Journal of Comparative Medicine
2017;27(8):66-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the feasibility of Cas9 microinjection in frozen-thawed pronuclear embryos, based on the model of pronuclear embryos of C57BL/6J mice by in vitro fertilization.Methods After fertilized mouse pronuclear embryos cultured in vitro, one-cell and 2-cell embryos were frozen using EFS20/40 cryopreservation tube.The next day recovered and then cultured.The recovery rate and survival rate of the one-cell and 2-cell embryos were compared.The frozen-thawed and fresh pronuclear embryos were injected with Cas9 mixture and injection buffer into the cytoplasm, and then cultivated to 2-cell embryos,and the survival rate and development rate of the 2-cell embryos were compared.Results The recovery rate of frozen-thawed one-cell embryos was 92.5%, the survival rate was 92.8%, the recovery rate of 2-cell embryos was 90.5% and the survival rate was 95.8%, showing no significant difference.Furthermore, the survival rate of fresh one-cell embryos after Cas9 injection was 92.7%, the survival rate of one-cell embryos of the blank group was 97.5%.While the survival rate of Cas9 injected frozen-thawed one-cell embryos was 82.6%, and that of the blank group was 92%,showing a significant difference between the frozen-thawed injected group and other groups(P < 0.05).The development rate of 2-cell embryos after Cas9 injection was not significantly different.Conclusions Frozen-thawed pronuclear embryos can be used for Cas9 microinjection.
