Expression differences and clinical significance of miR-29b,miR-24 and miR-200c in plasma of infants with PCG and normal
10.13389/j.cnki.rao.2017.0191
- VernacularTitle:原发性先天性青光眼(PCG)患儿与正常婴幼儿血浆中微小核糖核酸(miRNA或miR)-29b、miR-24、miR-200c的表达差异及临床意义
- Author:
Ling ZHENG
;
Guangqiang FENG
- Keywords:
microRNA-29 b;
microRNA-24;
microRNA-200 c;
plasma;
primary congenital glaucoma
- From:
Recent Advances in Ophthalmology
2017;37(8):755-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the expression differences of mircoRNA-29 b (miR-29b),microRNA? 24 (miR-24) and microRNA-200 c (miR-200c) in plasma of infants with primary congenital glaucoma (PCG) and normal,and analyze its clinical significance.Methods The expression quantity of microRNAs (miR-29b,miR-24,miR-200c) in plasma of PCG group (16 cases) and normal control group (49 cases) were detected by RT-PCR,and the relationship between their expression differences and severity of disease were analyzed.The diagnostic value of miRNAs for PCG was evaluated by receiver-operating characteristic curve (ROC).Results The expression quantity of miR-29b,miR-24,miR-200c in PCG group (0.31 ±0.19,0.17 ±0.16,0.55 ±0.18,respectively) were significantly lower than those in normal control group (1.18-±0.52,2.86 ±2.65,1.62 ± 0.76,respectively) (all P ＜ 0.05);The expression quantity of miR-24 and miR-29b in plasma was related to the severity of PCG,the more severe the disease,the lower the expression;ROC curve indicated that miR-24 and miR-29b had a higher diagnostic value for PCG disease than miR-200c.Conclusion Free miRNAs in plasma may be used as a new plasma markers for auxiliary diagnosis of PCG.
