Establishement of HPLC fingerprints of flavonoids in Ziziphi spinosae Semen and determination of two constituents
10.3969/j.issn.1001-1528.2017.05.022
- VernacularTitle:酸枣仁黄酮HPLC指纹图谱建立及2种成分测定
- Author:
Xu YANG
;
Ruixue RAN
;
Wei SONG
;
Wei QIAO
- Keywords:
"Ziziphi spinosae Semen;
flavonoids;
spinosin;
6""'-feruloylspinosin;
HPLC fingerprints"
- From:
Chinese Traditional Patent Medicine
2017;39(5):989-992
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC fingerprints of flavonoids in Ziziphi spinosae Semen and to determine the contents of two constituents.METHODS With spinosin as a reference peak,the HPLC fingerprints of ten batches of samples were established.The analysis of methanol extract of flavonoids in Ziziphi spinosae Semen was performed on a 30 ℃ thermostatic Agilent TC-C18column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ acetic acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS There were ten common peaks in the HPLC fingerprints,eight of which (vicenin-Ⅱ,glucosylvitexin,isospinosin,spinosin,6'-pyridyloylspinosin,6'-p-hydroxybenzoylspinosin,6'-feruloylspinosin and 6'-p-coumaroylspinosin) were identified.Spinosin and 6'-feruloylspinosin showed good linear relationships within the ranges of 15.00-40.00 μg and 5.00-14.00 μg,whose average recoveries (RSDs)were 100.5％ (1.6％) and 100.4％ (1.6％),respectively.CONCLUSION This accurate,simple and reliable method can be used for the quality control of flavonoids in Ziziphi spinosae Semen.
