Follicular stimulating hormone enhances Notch 1 expression in SK-OV-3 ovarian cancer cells.
10.3802/jgo.2010.21.2.119
- Author:
Young Han PARK
1
;
Su Jin KIM
;
Byung Hoon JEONG
;
Thomas J HERZOG
;
Jason WRIGHT
;
Jan KITAJEWSKI
;
Chae Chun RHIM
;
Pong Rheem JANG
;
Jung Bae KANG
;
Sung Ju KIM
Author Information
1. Department of Obstetrics and Gynecology, Hallym University Sacred Heart Hospital, Anyang, Korea. drparkyh@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Follicular stimulating hormone;
Notch 1;
Ovarian cancer
- MeSH:
Blotting, Western;
Cell Culture Techniques;
Cell Line;
Cell Migration Assays;
Cell Proliferation;
Coloring Agents;
Humans;
Ovarian Neoplasms;
RNA, Messenger;
RNA, Small Interfering
- From:Journal of Gynecologic Oncology
2010;21(2):119-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Notch is known as a transmembranous receptor family with four homologous forms - Notch 1, Notch 2, Notch 3, and Notch 4 and related to cell fate regulation and angiogenesis. The purpose is to investigate the effect of follicular stimulating hormone (FSH) on the Notch 1 expression and proliferation in ovarian cancer cells. METHODS: Human ovarian cancer cell line, SK-OV-3 and FSH were used. XTT cell proliferation and cell migration assay were carried out with FSH 100 mIU/mL and Notch 1 siRNA. Western blots and reverse transcriptase-polymerase chain reactions (RT-PCR) were carried out to determine the expression level of the Notch 1 protein and mRNA with FSH treatment in 0, 1, 5, 10, 100, 200, 300 mIU/mL concentrations. Immunofluorescent (IF) stains were performed in SK-OV-3 cell cultures with FSH 100 mIU/mL. Student-t tests were used in statistical analyses. RESULTS: The SK-OV-3 have Notch 1 receptors in their natural status. FSH stimulated SK-OV-3 cells in XTT cell proliferation and cell migration assays and notch 1 siRNA inhibited. The expression level of Notch 1 protein and mRNA were increased in a dose dependent pattern according to FSH concentrations compared to untreated cells. IF stains also showed brighter Notch1 expressions in the FSH treated cells compared to the control cells. CONCLUSION: FSH enhances proliferation & migration and Notch 1 signaling in SK-OV-3 cells. The Notch signaling probably supports one of the cell proliferating mechanisms of FSH in ovarian cancer cells.
