Simultaneous determination of five nucleosides in Houtoujun Tablets by RP-HPLC
10.3969/j.issn.1001-1528.2017.05.016
- VernacularTitle:RP-HPLC法同时测定猴头菌片中5种核苷
- Author:
Haiming TANG
;
Deqin WANG
;
Huiye ZHANG
- Keywords:
Houtoujun Tablets;
cytidine;
adenine;
uridine;
adenosine;
guanosine;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(5):964-967
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an RP-HPLC method for the simultaneous content determination of five nucleosides in Houtoujun Tablets (mycelia of Hericium erinaceum).METHODS The analysis of aqueous extract of this drug was performed on a 26 ℃ thermostatic Zorbax SB-Aq C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1％ phosphoric acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS Cytidine,adenine,uridine,adenosine and guanosine showed good linear relationships within the ranges of 0.007-0.169,0.005-0.115,0.032-0.797,0.032-0.792 and 0.004-0.103 μg,whose average recoveries (n =6) were 99.4％ (RSD =2.0％),98.3％ (RSD =1.8％),98.0％ (RSD =2.8％),101.4％ (RSD =1.9％) and 101.7％ (RSD =1.2％),respectively.CONCLUSION The contents of five nucleosides in Houtoujun Tablets from different manufacturers exhibit obvious differences,to which we should pay attention.
