CNTN-1 promotes the invasion andmigration of human esophageal cancer EC9706 cell line
- VernacularTitle:CNTN-1促进人食管癌EC9706细胞侵袭和迁移
- Author:
Yanfei CAO
;
Rui REN
;
Xianghui LUO
;
Ye YANG
;
Shuili WANG
- Keywords:
esophageal cancer;
CNTN-1;
invasion;
migration
- From:
Basic & Clinical Medicine
2017;37(9):1286-1291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of CNTN-1 on the invasion and migration of human esophageal cancer EC9706 cells and the possible mechanism.Methods The expression of CNTN-1 in human esophageal cancer EC9706 cells was measured by qPCR and Western blot.After transfection with CNTN-1 siRNA or CNTN-1, the cells were divided into control group, scrambled siRNA group, CNTN-1 siRNA group, pcDNA3.1-vector group and pcDNA3.1-CNTN-1 group.Cell proliferation, invasion and migration were respectively analyzed by BrdU assay and Transwell test.The expression of MMP-2 and MMP-9 were detected by qPCR and Western blot.Results The mRNA and protein expression of CNTN-1 were significantly upregulated in EC9706 cells.Compared with control, cell proliferation, invasion and migration, as well as the expression of MMP-2 and MMP-9 were significantly decreased by CNTN-1 siRNA, while they were increased by CNTN-1 overexpression (P<0.05).ConclusionsCNTN-1 can influence the invasion and metastasis of esophageal cancer cells through the regulation of the expression of MMP-2 and MMP-9.
