Establishment of Quality Standard for Extractum Glycyrrhizae Capsules
10.3969/j.issn.1008-049X.2017.09.036
- VernacularTitle:甘草浸膏胶囊质量标准的建立
- Author:
Hongda MA
;
Qiong WU
;
Changli WANG
;
Bei HU
;
Hui JIA
- Keywords:
Extractum glycyrrhizae capsules;
Quality standard;
Liquiritin;
Ammonium glycyrrhizinate;
HPLC;
TLC
- From:
China Pharmacist
2017;20(9):1651-1653
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality standard for two effective components in extractum glycyrrhizae capsules. Methods:Radix glycyrrhizae was identified by a TLC method. The contents of liquiritin and ammonium glycyrrhizinate in extractum glycyrrhizae capsules were determined by HPLC. An Inertsil C18 (150 mm × 4. 6 mm, 5μm) column was used. The mobile phase consisted of ace-tonitrile (A)-0. 2％ phosphoric acid (B) (0-8 min: 20％A-20％A;8-34 min: 20％A-50％A;34-35 min: 50％A-100％A;35-40 min:100％A-20％A) at a flow rate of 1. 0 ml·min-1 . The detection wavelength was at 237 nm under 25℃. Results: The spots in TLC were clear. Liquiritin showed a good linear relationship within the range of 0. 0020-0. 1000 mg·ml-1(r=0. 9995). The aver-age recovery was 100. 29％, and the RSD was 2. 94％(n=6). Ammonium glycyrrhizinate showed a good linear relationship within the range of 0. 0020-0. 1000 mg·ml-1(r=0. 9998). The average recovery was 101. 46％, and the RSD was 2. 33％(n=6). Conclu-sion:The method is simple,reliable and reproducible, which can be used for the quality control of the preparation.
