Effects of Jiedu Quyu Ziyin Decoction on TLR4 Signal Pathway in Lung, Spleen and Peritoneal Macrophagocyte of MRL/lpr Lupus Mice Treated by Prednisone
10.16466/j.issn1005-5509.2017.04.016
- VernacularTitle:解毒祛瘀滋阴方对强的松治疗的MRL/lpr狼疮鼠肺、脾、腹腔巨噬细胞TLR4信号通路的影响
- Author:
Guanqun XIE
;
Jinjun JI
;
Yongsheng FAN
- Keywords:
systemic lupus erythematosus;
MRL/lpr lupus mice;
macrophagocyte;
Jiedu Quyu Ziyin decoction;
glucocorticoid;
side effect;
Toll-like re-ceptor
- From:
Journal of Zhejiang Chinese Medical University
2017;41(4):318-322,344
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To observe the effect of Jiedu Quyu Ziyin decoction on TLR4 signaling pathway in macrophages of MRL/lpr lupus mice. [Method] MRL/lpr lupus mice were divided into four groups:model group, prednisone group, Jiedu Quyu Ziyin decoction group(hereinafter referred to as:Chinese medicine group) and prednisone plus Chinese medicine group (hereinafter referred to as:combination of Chinese and western medicine group). The mice were gavaged with saline, prednisone, Jiedu Quyu Ziyin decoction and prednisone added Jiedu Quyu Ziyin decoction for 4 weeks. Macrophages of lung, peritoneal and spleen were collected and the expression of related genes was detected by RT-PCR. [Result] TLR4 mRNA in lung macrophages, and TLR4 protein in splenic macrophages increased significantly(P<0.05) after the treatment of prednisone. The increased TLR4 protein in splenic macrophages was significantly decreased by combining with Jiedu Quyu Ziyin decoction(P<0.05). Prednisone can significantly reduce the TLR4 downstream molecules such as MyD88, IFN-α, iNOS mRNA expression in lung and peritoneal macrophages(P<0.05). The decreased MyD88 mRNA in lung macrophages was increased significantly by combining with Jiedu Quyu Ziyin decoction(P<0.05). [Conclusion] TLR4 signaling pathway is changed in macrophages after glucocorticoid administration in MRL/lpr lupus mice. Jiedu Quyu Ziyin decoction can reduce the abnormal glucocorticoid-induced TLR4 protein expression.
