Exosome-derived miR-20a inhibit apoptosis of TAM by targeting BCL2L11 in nasopharyngeal carcinoma
10.3969/j.issn.1671-8348.2017.06.001
- VernacularTitle:鼻咽癌外泌体miR-20a通过靶向BCL2L11基因抑制肿瘤相关巨噬细胞凋亡
- Author:
Longhui LV
;
Xiaoque HUANG
;
Xiaoming XIONG
;
Xu ZHANG
;
Zhihui YANG
;
Hongyan FANG
- Keywords:
nasopharyngeal neoplasms;
exosome;
miR-20a;
BCL2L11;
macrophages
- From:
Chongqing Medicine
2017;46(6):721-724,728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether exosome-derived microRNA of nasopharyngeal carcinoma suppresses apoptosis of tumor associated macrophage (TAM).Methods Target microRNAs and genes were determined by bioinformatics methods.Isolated exosomes were used to detect miR-20a expression by qRT-PCR.Furthermore,apoptosis index and proteins involved in apoptotic pathways were detected after miR-20a mimic and inhibitor transfection into macrophages.Results miR-20a expression was upregulated in isolated exosomes.miR-20a target gene was BCL2L11.MiR-20a overexpression could inhibit apoptosis of macrophages,meanwhile,apoptotic pathways related proteins Bim,caspase-9 and caspase-3 were significantly suppressed by miR-20a mimic(P＜0.05).Condusion miR-20a can suppress activation of Bim-caspase-9-casepase-3 and resulting in apoptotic inhibition of macrophages.
