Effect of Smad3 on cell migration of A549 and HeLa cells
10.3969.j.issn.1671-7856.2017.01.003
- VernacularTitle:Smad3对非小细胞肺癌A549细胞和宫颈癌HeLa细胞迁移的影响
- Author:
Rongfei WEI
;
Mengyuan LI
;
Xingjiu YANG
;
Ruimin ZHU
;
Damo XU
;
Ran GAO
- Keywords:
Smad3;
Overexpression vector;
RNAi;
SIS3;
Cell migration
- From:
Chinese Journal of Comparative Medicine
2017;27(1):11-15,21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Smad3 on cell migration of A549 and HeLa cells.Methods Primers for pCMV-Myc-Smad3 plasmid construction and siRNA targeting Smad 3 were designed and synthesized .pCMV-Myc-Smad3 plasmid was constructed with molecular cloning techniques .Overexpression of Smad 3 with Myc-tag or silencing of endogenous Smad3, and then scratch assay was used to detect the migration ability of A 549 and HeLa cells in vitro. Results pCMV-Myc-Smad3 plasmid was successfully constructed .Overexpression of Smad 3 significantly up-regulated the migration rate of A549 and HeLa cells.Conversely, in the same cells, silencing of endogenous Smad3 or treatment with Smad3 inhibitor, SIS3, down-regulated the migration rate .Conclusions Smad3 promotes cell migration of A549 and HeLa cells.
