Experimental study on abnormal expression of TPH2,DDC and MAO-A involved in depression-like behaviors of rats induced by CUS
10.3969/j.issn.1001-1978.2016.12.009
- VernacularTitle:CUS致大鼠抑郁行为涉及TPH2、DDC及MAO-A异常表达的实验研究
- Author:
Ting CUI
;
Wei XIE
;
Xiaoyan FU
;
Haiyan LI
;
Xinhui JIANG
;
Hongmei QIU
- Keywords:
CUS;
depression;
TPH2;
DDC;
MAO-A;
monoamine neurotransmitter
- From:
Chinese Pharmacological Bulletin
2016;32(12):1677-1682,1683
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of tryptophan hydroxylase-2 (TPH2),dopa-decarboxylase (DDC)and monoamine oxidase-A(MAO-A)in depression-like be-haviors induced by chronic unpredictable stress (CUS).Methods 30 male SD rats were randomly di-vided into model group(MG)and control group(CG). Rat depression model was developed by CUS for 28 consecutive days in a solitary condition.The depres-sion-like behaviors of rats were evaluated by open-field test(OFT)and forced-swimming test(FST).The real time PCR and Western blot test were used to determine the mRNA and protein expression of TPH2,DDC and MAO-A in rat telencephalon and hippocampus.Re-sults The movement scores of rats were obviously de-creased in OFT(P<0.01 ).The immobility time was obviously increased in FST (P <0.01 ).The mRNA and protein expressions of TPH2 and DDC were de-creased significantly (P <0.01,P <0.05 )and the MAO-A mRNA and protein expressions were increased significantly(P <0.01,P <0.05 )in telencephalon and hippocampus of MG rats, when compared with those in CG rats.Conclusion The TPH2,DDC and MAO-A in rat telencephalon and hippocampus were closely related with the depression-like behaviors of rats induced by CUS.
