Determination of Serum Concentration of Vitamin A in Healthy Volunteers and Hepatocirrhosis Patients by HPLC
10.6039/j.issn.1001-0408.2016.35.10
- VernacularTitle:HPLC法测定维生素A在健康受试者及肝硬化患者体内的血药浓度
- Author:
Fengmei XU
;
Meili YU
;
Rui SU
;
Wei LU
- Keywords:
HPLC;
Vitamin A;
Blood concentration;
Hepatocirrhosis
- From:
China Pharmacy
2016;27(35):4927-4929,4930
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the concentration determination of vitamin A(VitA)in human serum,and apply it in healthy volunteers and hepatocirrhosis patients. METHODS:After liquid-liquid extraction,serum sample was deter-mined by HPLC. Using VitA acetic ester as internal standard,the separation was performed on Kromasil C18 column with mobile phase consisted of methanol-water (98∶2,V/V) at the flow rate of 1 ml/min. The wavelength was set at 325 nm,and the column temperature was room temperature. The sample size was 20 μl. RESULTS:The linear range of VitA were 0.012 4-3.210 μg/ml(r=0.997 2,n=5),and the limit of quantification was 0.012 4 μg/ml. Inter-day and intra-day RSD ranged 1.66%-2.97%;sample re-coveries were 98.18%-99.56%;extraction recoveries were 89.59%-91.38%. Average blood concentration of VitA were(0.71±0.08)μg/ml in 24 healthy volunteers and (0.28 ± 0.06)μg/ml in 24 hepatocirrhosis patients. There was statistical significance in average blood concentration of VitA between healthy volunteers and hepatocirrhosis patients in different age groups (P<0.05). CONCLU-SIONS:The method is simple,rapid,sensitive and accurate,and can be used for the concentration determination of VitA in serum of healthy volunteers and hepatocirrhosis patients.
