Effect of SHBG gene on the apoptosis of human trophoblastic cells
10.11659/jjssx.02E016050
- VernacularTitle:SHBG 基因在人滋养细胞凋亡中的作用
- Author:
Xiaoxue XI
;
Siyu LIAN
;
Zhen JIN
;
Lei SUN
;
Qian SUN
;
Chong FENG
;
Yue WANG
;
Bao ZHANG
- Publication Type:Journal Article
- Keywords:
siRNA interference;
human trophoblastic cells;
SHBG gene;
apoptosis
- From:
Journal of Regional Anatomy and Operative Surgery
2016;25(10):711-714,715
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sex hormone-binding globulin (SHBG)gene in the apoptosis of human trophoblastic cells.Methods The siRNA specific-targeting SHBG gene was transfected into human trophoblastic cells and they were divided into six groups:trophoblasts without transfection in normal control groups(group Ⅰ);transfect liposome in blank control groups(group Ⅱ);transfect nonspecific siRNA in negative control groups(group Ⅲ);transfect SHBG siRNA-Ⅰ,SHBG siRNA-Ⅱ,SHBG siRNA-Ⅲ respectively in trans-fection group(group Ⅳ,Ⅴ,Ⅵ).Hoechst 33258 dying method was used to detect cell apoptosis.SHBG and Caspase-3 mRNA profiling and the level of SHBG and caspase-3 protein were detected by real-time PCR and Western blot.Results There was no statistical significant difference in the gene expression and protein level of SHBG and caspase-3 in group Ⅰ,Ⅱ and Ⅲ (P >0.05).In Ⅳ,Ⅴ and Ⅵ group,there was no statistical significant difference in the expression level of SHBG and caspase 3 (P >0.05).Compared with group Ⅰ,Ⅱ and Ⅲ,the a-mount of SHBG gene expression decreased obviously,the caspase-3 mRNA and protein level increased obviously and the trophoblast cell ap-optosis increased markedly (P <0.05).Conclusion Through siRNA interference technology can reduce SHBG gene expression in human trophoblastic cells,and it can lead to excessive apoptosis of human trophoblasts cells.
