PPARγexpression in rat lung tissue after ventilator-induced lung injury
10.11659/jjssx.1672-5042.201406004
- VernacularTitle:机械通气致大鼠肺损伤时肺组织PPARγ表达的变化
- Author:
Xiuqin WANG
;
Kaiguo WANG
;
Hongmin LUO
;
Hao LI
;
Baosheng WANG
;
Peimin WANG
- Publication Type:Journal Article
- Keywords:
lung injury;
mechanical ventilation;
peroxisome proliferator activated receptor-γ(PPARγ)
- From:
Journal of Regional Anatomy and Operative Surgery
2014;(6):583-585,586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of PPARγ expression in ventilator-induced lung injury rats and explore the role of PPARγ in the pathogenesis of ventilator-induced lung injury. Methods Sixty male Sprague-Dawley rats were randomly divided into 3 groups ( n=21 each ):group N received large tidal volume with mechanical ventilation ( Vt=12 mL/kg);group C received lower tidal volume with mechanical ventilation ( Vt=6 mL/kg);group R received room air without mechanical ventilation. Rats in every group were randomly divided into 3 subgroups respectively by 1,4 and 8 h. The samples of lung were collected at 1,4 and 8 h after ventilation. Lung pathological examina-tion, total protein and white blood cells in bronchoalveolar fluid and wet-to-dry weight were detected. The exoressions of PPARγmRNA were detected by RTPCR;PPARγ protein in lung tissues was detected by western bolt. Result After 4 and 8 h ventilation in group N,total pro-tein and WBC in bronchoalvelor fluid,W/D were markedly higher than those of group C and R (P <0. 01). While PPARγ mRNA and PPARγ protein were decreased than those of group C and R (P<0. 01). There were no difference after 1 h ventilation in three group (P>0. 05). Conclusion PPARγmRNA and protein expressions in the rats lung tissue of ventilator-induced lung injury were decreased and as-sociated with inflammation and damage of lung tissue.
