Laminar shear stress result in maintaining the quiescence of MSCs

VernacularTitle:剪切力对间充质干细胞的细胞休眠状态的诱导作用
Author: Wei LUO ; Feng LI ; Wei XIONG ; Shichang GAO
Publication Type:Journal Article
Keywords: mesenchymal stem cells; shear stress; cell cycle arrest; apoptosis
From: Journal of Regional Anatomy and Operative Surgery 2015;(2):123-125,126
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of laminar shear stress ( LSS) on the proliferation and apoptosis of mesenchymal stem cells ( MSCs) . Methods The influences of laminar shear stress ( the physiological levels) on MSCs were studied. DNA synthesis and cell cycle were measured,to detect the cellular proliferation. The apoptosis-related gene expression ( Bcl-2/Bax) was examined to determine the effect of LSS. Results The percentage of MSCs’ proliferation rate was reduced by the fluid shear stress. Furthermore,it was detected that LSS exerted a potent suppression effect on MSC apoptosis. Conclusion These data revealed a critical role of LSS in maintaining the quies-cence of MSCs.