Determination of Zolpidemtartrate in Plasma by SPE-HPLC
- VernacularTitle:固相萃取结合高效液相色谱法测定人血浆中酒石酸唑吡坦的浓度
- Author:
Junping GUO
;
Anjin ZHANG
;
Minfang HU
- Publication Type:Journal Article
- Keywords:
HPLC;
Solid-phase extraction;
Zolpidemtartrate;
Plasma concentration
- From:
China Pharmacist
2016;19(4):788-789,790
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of zolpidemtartrate in human plasma by SPE-HPLC. Methods:The samples were extracted by solid phase with a Hypersil BDS C18 column and determined by an internal standard method. The mobile phase was methanol:acetonitrile:water (45 ∶45 ∶110), the flow rate was 1. 0 ml·min-1, the column temperature was 35℃, and the detection wavelength was 254nm. Results:The linear relationship of zolpidemtartrate was within the range of 0. 511 5-4. 092 0 μg· ml-1 . The relative recovery was greater than 90%. The intra-and inter-day RSDs were all less than 10%. Conclusion:The method is quite simple, quick and accurate with little interference, which is suitable for the clinical determination of zolpidemtartrate.
