Modulation effect of chromosome kinesin protein KIF4A on cisplatin resistance of A549/DDP cells
10.3760/cma.j.issn.1673-4181.2015.06.002
- VernacularTitle:染色体驱动蛋白KIF4A参与调控肺癌细胞顺铂耐药
- Author:
Chenghua FU
;
Xiaoqing HU
;
Shuangshuang LIANG
;
Meichao JI
;
Zhixiong DONG
;
Changjun ZHU
- Publication Type:Journal Article
- Keywords:
Lung cancer;
KIF4A;
Cisplatin resistance
- From:
International Journal of Biomedical Engineering
2015;38(6):327-330,335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the process that chromosome kinesin KIF4A promote cisplatin resistance in lung cancer cells.Methods Reverse transcription PCR (RT-PCR) and Western Blot experiments were performed to analyze the expression of KIF4A in lung cancer cells A549 and cisplatin (DDP) resistant cells A549/ DDP.Cell transfection, RNA interference (RNAi) experiments and thiazolyl blue tetrazolium bromide (MTT) assays were carried out to examine cell proliferation of A549 cells with overexpression of exogenous KIF4A and A549/DDP cells with depletion of endogenous KIF4A after cisplatin treatment.Results Expression of KIF4A in A549/DDP cells was higher than that in A549 cells.With overexpression of exogenous KIF4A, A549 cells displayed drug resistance to cisplatin.On the contrary, depletion of endogenous KIF4A in A549/DDP cells resulted in cisplatin sensitivity.Conclusions Chromosome kinesin KIF4A involves in the regulation of cisplatin resistance in lung cancer cells and KIF4A may be a potential and effective new biological target for treatment of lung cancer cisplatin resistance.
