Study on HPLC fingerprint of radix euphorbiae fischerianae
10.3969/j.issn.1673-4130.2016.04.006
- VernacularTitle:狼毒药材HPLC指纹图谱研究
- Author:
Dehong DENG
;
Ju HU
- Publication Type:Journal Article
- Keywords:
Radix Euphorbiae Fischerianae;
high performance liquid chromatography;
fingerprints;
quality control
- From:
International Journal of Laboratory Medicine
2016;37(4):447-448,451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish fingerprint of Radix Euphorbiae Fischerianae by high performance liquid chromatography (HPLC) .Methods The samples were separated on Hypersil ODS(250 mm × 4 .6 mm ,5 μm) column with gradient mobile phase of acetic-water ,the column temperature was 20 ℃ with the flow rate of 1 .0 mL/min and UV detection wavelength was 210 nm .the sample injection was 20 μL .Results 15 samples of different origin Radix Euphorbiae Fischerianae were detected and each chroma-tographic peak was separated well with conforming to the requirements of fingerprint by calibrating the 15 peaks .15 common peak retention time RSD ,relative peak area RSD were 3 .7% ,3 .6% or less respectively ;The chromatographic peak separation degree was better ,similarity was higher ,15 batches of different sources of stellera medicinal materials ,in addition to the Guangxi nanning medi-cine material similarity was 0 .858 ,other origin medicine material similarity above 0 .95 .Have reached the technical requirements of traditional Chinese medicine (TCM ) fingerprint .Conclusion The method is accurate ,reliable ,and the HPLC fingerprint shows good repeatability ,which can be used for one of the quality control of Radix Euphorbiae Fischerianae .
