Effects of Allicin on myocardial fibrosis and TLR4/NF-κB pathway

VernacularTitle:大蒜素对心肌纤维化的影响及对TLR4／NF-κB信号通路的作用
Author: Jingsheng LAN ; Zhen ZHANG ; Wei LUO ; Ziqing QIN ; Yanying ZHAO
Publication Type:Journal Article
Keywords: Allicin; Cardiac fibroblasts; Toll like receptor-4/Nuclear factor-κB
From: Chinese Journal of Immunology 2016;32(4):500-503,508
CountryChina
Language:Chinese
Abstract: Objective:To observe the effect of Allicin in cardial fibroblasts (CFs) proliferation and Collagen secretion,and to explore its role on TLR4/NF-κB signal pathway.Methods:CFs of neonatal Wistar rats were isolated and cultured ,then was stimulated with AngⅡ.CFs proliferation was measured by thiazolyl blue ( MTT) assay.The expression of collagenⅠ,collagenⅢ was measured by ELISA.mRNA expression of TLR4 and NF-κB were detected by reverse transcription-polymerase chain reaction ,protein expression of TLR4 and NF-κB were detected with Western blot.Results: Allicin could reduced MTT value of cardial fibroblasts ( P<0.01 ) , and inhibited expression of collagenⅠ,collagenⅢ(P<0.01),which in a dose-dependent manner.Allicin could reduced mRNA expression of TLR4 and NF-κB and protein expression of TLR 4 and NF-κB in CF induced by Ang Ⅱ ( all P<0.01 ) .Conclusion: Allicin can inhibit Myocardial fibrosis ,which mechanism is possible by inhibiting TLR 4/NF-κB signal pathway.