Effects of Cytokine Milieu Secreted by BCG-treated Dendritic Cells on Allergen-Specific Th Immune Response.
10.3346/jkms.2004.19.5.640
- Author:
Youngil I KOH
1
;
Inseon S CHOI
;
Je Jung LEE
Author Information
1. Department of Allergy and Hematology, Chonnam National University Medical School and Research Institute of Medical Science, Gwangju, Korea. yikoh@chonnam.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Asthma;
Cytokines;
Dendritic Cells;
Mycobacterium bovis;
T-lymphocytes
- MeSH:
Antigens, Dermatophagoides/*immunology;
Asthma/*immunology;
Cells, Cultured;
Coculture Techniques;
Culture Media;
Cytokines/*immunology/secretion;
Dendritic Cells/cytology/*immunology/secretion;
Humans;
Hypersensitivity/immunology;
Interferon Type II/immunology/secretion;
Interleukin-10/immunology/secretion;
Interleukin-12/immunology/secretion;
Interleukin-5/immunology/secretion;
Lymphocyte Activation/immunology;
Mycobacterium bovis/*immunology;
Research Support, Non-U.S. Gov't;
Th2 Cells/cytology/immunology/secretion;
Up-Regulation/immunology
- From:Journal of Korean Medical Science
2004;19(5):640-646
- CountryRepublic of Korea
- Language:English
-
Abstract:
Bacillus Calmette-Guerin (BCG) is reported to suppress Th2 response and asthmatic reaction. Dendritic cells (DCs), the major antigen-presenting cells, infections with BCG are known to result in inducing various cytokines. Thus, DCs are likely to play a role in the effects of BCG on asthma. This study aims at investigating that cytokine milieu secreted by BCG-treated DCs directly enhances allergen-specific Th1 response and/or suppresses Th2 response in allergic asthma. DCs and CD3+ T cells were generated from Dermatophagoides farinae-sensitive asthmatics. DCs were cultured with and without BCG and subjected to flow cytometric analysis. IL-12 and IL-10 were determined from the culture supernatants. Some DCs were cocultured with T cells in the presence of D. farinae extracts after adding the culture supernatants from BCG-treated DCs, and IL-5 and IFN-gamma were determined. BCG-treated DCs enhanced significantly the expressions of CD80, CD86, and CD40, and the productions of IL-12 and IL-10. Addition of culture supernatants from BCG-treated DCs up-regulated production of IFN-gamma by T cells stimulated by DCs and D. farinae extracts (p<0.05), but did not down-regulate production of IL-5 (p>0.05). The cytokine milieu secreted by BCG-treated DCs directly enhanced allergen-specific Th1 response, although did not suppress Th2 response.
