Effect of Baicalin on LPS-induced Apoptosis and Cell Migration of Intestinal Epithelial Cells
10.13359/j.cnki.gzxbtcm.2015.01.027
- VernacularTitle:黄芩苷对脂多糖诱导的肠道上皮细胞凋亡及迁移能力的影响
- Author:
Jian CHEN
;
Ling HUANG
;
Yan LI
;
Siying LIU
;
Zaoyuan KUANG
- Publication Type:Journal Article
- Keywords:
Baicalin/pharmacology;
Intestinal epithelial cells/pathology;
Apoptosis;
Cell migration;
Cell culture
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(1):126-129,135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of baicalin on the apoptosis and cell migration of intestinal epithelial cells 6 (IEC-6) induced by lipopolysaccharide (LPS) . Methods LPS was adopted to establish LPS-induced cells injury model. Methyl thiazolyl tetrazolium ( MTT) assay and Elisa kits were used to evaluate the effect of LPS on the tumor necrosis factor alpha ( TNF-α) and interleukin 6 ( IL-6) concentrations of IEC-6. Hoechst 33258 staining and flow cytometry were used to evaluate the effect of baicalin ( in the dosage of 2.5, 5.0, 10.0 μg/mL ) on the cell apoptosis. Cell migration was observed and calculated on a wounding model of IEC-6 cells induced by a pipette tip. Results Compared with the control group, the survival rate of IEC-6 in 1.0 μg·mL-1 LPS group was much lowered, TNF-α and IL-6 concentrations were significantly increased, the apoptotic rate of IEC-6 was increased and the cell migration activity was significantly decreased ( P<0.05) . Compared with the model group, IEC-6 apoptosis was relieved to various degrees in baicalin groups, the apoptotic rate of IEC-6 was obviously decreased (P<0.05) and cell migration activity was increased significantly (P<0.01) in 10.0 μg·mL-1 baicalin group. Conclusion Baicalin has the capacity of protecting IEC-6 from LPS-induced injury and increasing cell migration activity.
