Inhibitory effect of isobavachalcone on migration and invasion of Tca8113 cells and its mechanism
10.3969/j.issn.1001-1978.2015.12.022
- VernacularTitle:补骨脂乙素对 Tca8113细胞迁移及侵袭的抑制作用及其机制
- Author:
Yi SHI
;
Weizhong WU
;
An HUO
;
Wei ZHOU
;
Zhitu ZHU
- Publication Type:Journal Article
- Keywords:
isobavachalcone;
tongue squamous cell carcinoma;
proliferation;
migration;
invasion;
mecha-nism
- From:
Chinese Pharmacological Bulletin
2015;(12):1741-1744,1745
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the inhibitory effect of isobavachalcone (IBC)on migration and invasion of
tongue squamous cell carcinoma Tca81 1 3 cells and its possible mechanism.Methods Tca81 1 3 cells were
treated in different concentrations of IBC in vitro.Cell proliferation was detected by MTT;Wound healing as-say and Transwell chamber assay were used to detect the ability of cell migration and invasion;Western blot was applied to detect the expression of Akt,p-Akt, MMP-2 and MMP-9 proteins.Results IBC could in-hibit the proliferation of Tca81 1 3 cells in a concentra-tion-and time-dependent manner.IBC can reduce cell migration and invasion.Western blot showed that IBC could an decrease the expression of p-Akt,MMP-2 and MMP-9 proteins in a concentration-dependent manner.
However,the level of Akt was not affected by the con-centration of IBC treatment.Conclusion IBC could inhibit the proliferation, migration and invasion in Tca81 1 3 cells and its mechanism may be associated with the down-regulation of MMP-2 and MMP-9 pro-teins and the inhibition of phosphorylation of upstream Akt.
