Detection of immunoglobulin and TCR gene rearrangements by PCR using BIOMED-2 multi-plex protocols in T lymphoblastie lymphoma and acute lymphoblastic leukemia
10.13315/j.cnki.cjcep.2015.10.013
- VernacularTitle:BIOMED-2引物系统检测 T 淋巴母细胞性淋巴瘤/急性淋巴细胞白血病中Ig/TCR基因重排
- Author:
Xinyan PAN
;
Qiang FENG
;
Guiyun LI
;
Changshao YANG
;
Julun YANG
;
Li WANG
- Publication Type:Journal Article
- Keywords:
T lymphoblastic lymphoma;
acute lymphoblastic leukemia;
BIOMED-2;
gene rearrangement;
Ig/TCR gene
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(10):1131-1134,1139
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the sensitivity of BIOMED-2 primer system in T lymphoblastic lymphoma ( T-LBL) and acute lym-phoblastic leukemia ( ALL) patients immunoglobulin ( Ig) and T-cell receptor ( TCR) gene rearrangement, and to analyze the co-rear-rangement pattern. Methods Amplification of rearranged Ig and TCR gene was performed in standard PCR in 35 T-LBL/ALL pa-tients. PCR products were analyzed by heteroduplex and polyacrylamide gel electrophoresis. Results 16 cases (45. 7%) of 35 sam-ples were detected to have TCR gene rearrangements, including 6 cases (37. 5%) of TCRβgene monoclonal rearrangements, 4 cases (25. 0%) of TCRγ gene monoclonal rearrangements, 3 cases (18. 8%) of TCRβ and TCRγ gene double rearrangements, 2 cases (12. 5%) of TCRδ gene monoclonal rearrangements and 1 case (6. 3%) of TCRγand TCRδgene double rearrangements were detec-ted. 4 cases (11. 4%) of 35 samples detected to have clonal immunoglobulin and TCR gene rearrangements. 11 cases (39. 3%) of 28 T-LBL patients were detected to have TCR gene rearrangements, 6 cases (85. 7%) of 7 T-ALL have TCR gene rearrangements. Con-clusions BIOMED-2 multiplex PCR analysis strategy is a useful technique in the T-LBL patients.
