Hemocyanin is an important high-molecular-weight allergen of Eriocheir sinensis
10.3969/j.issn.1000-484X.2015.10.017
- VernacularTitle:血蓝蛋白是中华绒鳌蟹重要的高分子量过敏原
- Author:
Yingying ZHANG
;
Lina ZHU
;
Shaoshen LI
;
Liying HOU
;
Huiqiang LI
- Publication Type:Journal Article
- Keywords:
Eriocheir sinensis;
Specific sIgE;
Hemocyanin;
MALDI-TOF/TOF analysis;
Allergen
- From:
Chinese Journal of Immunology
2015;(10):1375-1379,1388
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prove hemocyanin is an important high-molecular-weight allergen from Eriocheir sinensis.Methods:The proteins were extracted from Eriocheir sinensis tissue with lysate extraction method .The protein components were analyzed by SDS-PAGE,two-dimensional electrophoresis and Western blot.Serum IgE from allergic donors identified a candidate protein,which was char-acterized by MALDI-TOF/TOF.The immune property of the candidate protein was tested using Dot-blot.Results: By SDS-PAGE and two-dimensional electrophoresis analysis,we prove that the native protein components were completely.According to the Western blot result,at least 18 components could react with the positive serum.Among them, two 70-80 kD proteins had the same isoelectric point.So,may be they were the same protein.MALDI-TOF/TOF analysis results showed that the suspected proteins were hemocyanin.A sensitization frequency of 61%was observed in Eriocheir sinensis patients by Dot-blot.Conclusion:Hemocyanin was identified as an important high-molecular-weight allergen from Eriocheir sinensis.
