Biological activity and application study of a monoclonal antibody against human Tim-3
10.7644/j.issn.1674-9960.2014.08.012
- VernacularTitle:Tim-3抗体的生物学活性及应用研究
- Author:
Chunmei HOU
;
Xiaoling LANG
;
Yan XIAO
;
Mingke ZHENG
;
Xingwei JIANG
;
He XIAO
;
Guojiang CHEN
;
Renxi WANG
;
Jiannan FENG
;
Gencheng HAN
;
Beifen SHEN
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Tim-3;
antibodies,monoclonal;
L3D;
U937 cells;
inflammatory factors;
sepsis
- From:
Military Medical Sciences
2014;(8):617-620,625
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a human Tim-3 specific monoclonal antibody and evaluate its biological activity and possible use in clinical diseases associated with dysregulated Tim-3 expression .Methods The BALB/c mice were immu-nized by conventional method, and positive clones were used to develop anti-human Tim-3 antibody, the binding and neutralization activities of which in vitro and in vivo were investigated.Results ①A monoclonal antibody (clone L3D) which could specifically bind to human Tim-3 protein in ELISA assay was obtained and the subtype of the monoclonal antibody was IgG2a .②Flow cytometry indicated that the monoclonal antibody could bind to Tim-3 expressed in human U937 cells.This antibody also showed a cross activity to mice′Tim-3.③The monoclonal antibody inhibited the apoptosis of THP1 cells induced by Gal-9, the ligand of Tim-3.④Injection of Tim-3 antibody exacerbated sepsis in mice as marked by the decreased survival rate and increased expression of pro-inflammatory cytokines .Conclusion An anti-human Tim-3 monoclonal antibody is successfully obtained.The excellent binding and neutralization activities of this antibody enable it to be widely used in clinical diseases associated with deregulated Tim-3 expression .
