Effects and mechanisms of 2-methoxyestradiol on the expression of apoptosis-related genes in K562 leukemia cells
10.3760/cma.j.issn.1009-9921.2009.06.002
- VernacularTitle:2-甲氧基雌二醇对白血病K562细胞凋亡相关基因表达的影响及其机制
- Author:
Dongliang LI
;
Jing ZHANG
;
Ling PAN
;
Lanfen HOU
;
Zhiwei WANG
;
Xiao GUO
- Publication Type:Journal Article
- Keywords:
K562 cells;
2-methoxyestradiol;
Apoptosis;
Leukemia,myeloid,chronic
- From:
Journal of Leukemia & Lymphoma
2009;18(6):323-326,330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 2-methoxyestradiol( 2-ME) on apoptosis of K562 cells and its mechanisms. Methods The K562 cells were cultured and divided into three groups. The control group: K562 cells were cultured without 2-ME treatment. The experimental group: K562 cells were cultured in the medium containing different concentrations of 2-ME (1, 2, 4, 8 and 16 μmol/L) for 36 h. The negative control group: K562 cells were replaced by water without RNase in the medium containing different concentrations of 2-ME for 36 h. The apoptosis rate, the protein and its mRNA expression of Caspase-3 and XIAP, the activity of superoxide dismutase (SOD) and reactive oxygen species (ROS) of K562 cells wasdetected by TUNEL, flow cytometry (FCM), half-quantitative RT-PCR and xanthenes oxidized enzyme assay,respectively. Results After treated with 2-ME at different concentrations for 36 hours, in the specified concentration range, 2-ME induced apoptosis of K562 cells in a concentration-dependent manner. The possible functional mechanism of 2-ME was to up-regulate Caspase-3 but down-regulate XIAP mRNA expression, and increase ROS activity but decrease SOD activity. Conclusion 2-ME can induce apoptosis of K562 cells in a concentration-dependent manner and indicate its promising potential in the treatment of chronic myeloid leukemia(CML) patients.
