Histopathological changes of hippocampus after acute epilepsy induced by pentylenetetrazole in rats

VernacularTitle:戊四唑急性癫痫模型海马病理组织的变化
Author: Xiaohu LIU ; Shaojie XIANG ; Yue QI ; Miao LI ; Xinpei LI ; Li MENG ; He CHEN ; Dong JIA
Publication Type:Journal Article
Keywords: pentylenetetrazole; Nissl staining; caspase-3; AIF; epilepsy; rat
From: Chinese Pharmacological Bulletin 2015;(4):514-517,518
CountryChina
Language:Chinese
Abstract: Aim To observe histopathological changes of hippocampus after acute epilepsy induced by penty-lenetetrazole (PTZ)in rats.Methods Five groups as control group,PTZ-induced 24 hours(h)group,PTZ-induced 72 hours group,PTZ-induced 1 20 hours group and PTZ-induced 1 44 hours group were designed.PTZ (64 mg·kg -1 )was administered with a single intrap-eritoneal injection for generalized tonic-clonic sei-zures in the current experiment.Control and PTZ trea-ted animals were sacrificed after specific time points. Brain was dissected out and then evaluated for neuro-pathological changes using Nissl staining and immuno-histochemical technique.Results In this study PTZ-induced hippocampal neuron status apoptosis occurred at 24 hours and was sustained for 1 44 hours after status epilepticus.Whereas,activated caspase-3 and AIF ap-peared at 24 hours and were sustained for 1 44 hours af-ter status epilepticus.Conclusion The results of this study show that the significant histopathological chan-ges of hippocampus appear in the vicinity of 1 20 hours after intraperitoneal injection of pentylenetetrazole.