Tongxinluo capsule inhibits oxidative stress in diabetic peripheral neuropathy mice by inhibiting the activity of p-p38 MAPK
10.3969/j.issn.1001-1978.2015.05.026
- VernacularTitle:通心络胶囊抑制p38 MAPK磷酸化抑制糖尿病周围神经病变小鼠氧化应激
- Author:
Chao WANG
;
Huixin ZHANG
;
Hanying XING
;
Xing WANG
;
Min LIU
;
Zhe ZHANG
- Publication Type:Journal Article
- Keywords:
Tongxinluo capsule;
diabetic peripheral neuropathy;
oxidative stress;
MAPK;
p38 MAPK;
KK/Upj-Ay mice
- From:
Chinese Pharmacological Bulletin
2015;(5):726-729,730
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Tongxin-luo capsule on oxidative stress in diabetic peripheral neuropathy (DPN )mice and its mechanisms.Meth-ods KK/Upj-Ay mice were divided into model, Tongxinluo low-dose group, Tongxinluo middle-dose group and Tongxinluo high-dose group.C57BL/6 mice were selected as control group.Mice were given drugs intragastrically for 12 weeks.Paw withdrawal latency, motor nerve conduction velocity (MNCV)were detec-ted.Activity of superoxide dismutase (SOD),gluta-thione peroxidase (GSH-Px)and content of malondial-dehyde (MDA)in blood were detected by colorimetric method.The expression of heme oxygenase-1 (HO-1 ),γ-glutamyl cysteine synthetase (γ-GCS ) of sciatic nerve was examined by real time PCR and Western blot.The protein expression of p38 MAPK,p-p38 MAPK,JNK,p-JNK,ERK,p-ERK was examined by Western blot.Results Compared with model group, paw withdrawal latency was increased and MNCV was faster in Tongxinluo group (P <0.05 ,P <0.0 1 ). SOD and GSH-Px contents significantly increased, MDA content decreased (P <0.01 ).HO-1,γ-GCS mRNA and protein expression significantly increased (P<0.05,P <0.01 )in Tongxinluo group.p-p38 MAPK protein expression decreased in Tongxinluo group (P<0.05 ).Conclusion Tongxinluo can in-hibit oxidative stress in DPN of mice via suppressing the phosphorylation of p38 MAPK.
