Establishment of a new congenic inbred mouse strain named Foxn1nu.B6-CAG-EGFP/SU
10.3969/j.issn.1671.7856.2015.001.010
- VernacularTitle:同源导入近交系绿色荧光裸小鼠 Foxn1 nu ． B6－CAG－EGFP／SU 的建立
- Author:
Yanhua SHEN
;
Qilong WANG
;
Xingliang DAI
;
Jinsheng CHEN
;
Jun DONG
;
Qing LAN
;
Qiang HUANG
- Publication Type:Journal Article
- Keywords:
Green fluorescent protein;
Transgenic nude mice;
Nude mouse;
Inbred strain
- From:
Chinese Journal of Comparative Medicine
2015;(1):55-58,65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new congenic inbred mouse strain carrying and expressing EGFP and Foxn1nugene for cancer research including human glioma as well .Methods According to criterion of GB14923-2010, the male Foxn1nu nude mice backcross the female C57BL/6-Tg (CAG-EGFP) transgenic mice for 10 times, Then identify the phenotype using the methods and equipment as below: fluorescent flashlight and matching glasses; multifunction vivo imager; fluorescence microscopy.Results The congenic inbred mouse strain named Foxn1nu.B6-CAG-EGFP/SU ( Soochow University ) .All the 14 biochemical loci are homozygous and same with Balb/c mouse in addition to the Pep3 loci (“b” type instead of “a” type).Peripheral blood lymphocyte count shows the lymphocytes occupy 15%of nucleated cells;T lymphocytes occupy 0.3%, meet the requirement of inbred strain of EGFP nude mice .Conclusions Established a new congenic inbred strain -Foxn1nu.B6-CAG-EGFP/SU which both express EGFP stably, and own immunodeficiency with lack of T lymphocytes .The phenotype “b” of biochemical loci “Pep3” is the unique characteristic that distinguish SU to Foxn1nu.
