LC-MS/MS method for simultaneous determination of three monoamine neurotransmitters in rat plasma of CUMS
10.3969/j.issn.1001-1978.2015.02.025
- VernacularTitle:LC-MS/MS法同时测定CUMS大鼠血浆中3种单胺类神经递质
- Author:
Yuxin ZANG
;
Bingting SUN
;
Wenzhu ZHAO
;
Na RONG
;
Guoliang DAI
;
Wenzheng JU
;
Hengshan TAN
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
CUMS;
rats;
plasma;
5-HT;
DA;
NE;
derivatization
- From:
Chinese Pharmacological Bulletin
2015;(2):273-277,278
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a LC-MS/MS method for determination of 5-HT, NE, DA and observe the con-centration of 5-HT, NE, DA in rat plasma of CUMS. Methods Twenty-two male SD rats were divided into control group and model group. Model group was given 9 kinds chronic unpredictable mild stimulating factors every day. 21 days later, behavior and orbital blood were measured before and after modeling. Using benzo-yl chloride as a pre-column derivatization reagent, three analytes and IS were derivatized before LC-MS/MS detection. Change in three kinds of neurotransmit-ter concentration was measured in rat plasma before and after modeling. Results After modeling, com-pared with control group, the weight of rats in model group was declined significantly ( P<0. 05 ) . Horizon-tal scores, vertical scores and sugar consumption were declined significantly ( P <0. 01 ) . Calibration curves of 5-HT, NE, DA were linear between 1. 47 ~752, 1. 75 ~898 , 2. 05 ~1 053 μg · L-1 and LOQ were 1. 47, 1. 75, 2. 046μg·L-1 ,respectively. The recov-ery of 5-HT, NE, DA from plasma was over than 70%, and RSD of inter-day and intra-day assay was limited in 15%. Compared with control group, the con-centration of 5-HT, NE, DA in rat plasma of model group was declined to ( 3. 99 ± 1. 21 ) , ( 6. 24 ± 1. 94), (6. 07 ± 1. 98) μg·L-1(P <0. 01). Con-clusion After making CUMS model of depression, three kinds of neurotransmitters in rat plasma are de-creased.
