Effect of bone marrow mesenchymal stem cell transplantation on silicosis fibrosis in different time windows in rats
10.3969/j.issn.1000-484X.2015.02.012
- VernacularTitle:不同时间骨髓间充质干细胞移植对大鼠矽肺纤维化形成的影响
- Author:
Huixing ZHU
;
Junling GAO
;
Manman ZHAO
;
Ran LI
;
Yanxia TIAN
;
Xin WANG
;
Juan ZHANG
;
Jianzhong CUI
- Publication Type:Journal Article
- Keywords:
Silicosis;
BMSCs;
MMP-9;
Collagen type Ⅰ;
Collagen type Ⅲ
- From:
Chinese Journal of Immunology
2015;(2):193-196,209
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of bone marrow mesenchymal stem cell transplantation on silicosis fibrosis in different time windows in rats.Methods:BMSCs were isolated and cultured from male 3-week-old SD rats in vitro.Fifty healthy female SD rats were randomly divided into 5 groups:control group,silicosis model group,early treatment group,middle treatment group,late treatment group(n=10).The silicosis model was made by one-time infusion of silica dust suspension using the non-exposed tracheal in-tubation(50 μg/ml),and 1 d,14 d,28 d of BMSCs were given for intervention therapy (1 ×106 ml-1 ).Rats in each group were sacrificed 14 days after treatment.The BMSCs identified by flow cytometry;the morphological changes of the lung tissues were observed by HE staining;the expression of MMP-9,collagen type Ⅰ and collagen type Ⅲ were detected by immunocytochemistry and Western blot analysis.Results: BMSCs in early silicosis ( 1 d ) and the middle silicosis ( 14 d ) compared to silicosis model group can significantly alleviate the pathological process of silicosis fibrosis (P<0.05),BMSCs in late silicosis (28 d) treatment had no significant effect(P>0.05).Conclusion:Exogenous BMSCs transplantation on rat silicosis early pathological processes play a role in delaying , late treatment effect is not obvious.
