miR-100 expression in hepatocellular carcinoma tissues with fluorescent in situ hybridization

VernacularTitle:荧光原位杂交法检测肝细胞癌组织中miR-100的表达
Author: Hui ZHANG ; Yangkun WANG ; Wuling ZHU
Publication Type:Journal Article
Keywords: hepatocellular neoplasms; miR-100; differentiation; fluorescence in situ hybridization
From: Chinese Journal of Clinical and Experimental Pathology 2015;(2):184-185,186
CountryChina
Language:Chinese
Abstract: Purpose To investigate the expression and clinical significance of miR-100 in hepatocellular carcinoma ( HCC) . Methods LNA-modified miR-100 fluorescent probe was used to detect the formalin-fixed and paraffin-embedded tissues of 29 cases of HCC, while 10 cases of normal human liver tissues were used as the control group. Results Analysis of fluorescence in situ hybridization ( FISH) showed that, in all normal tissues miR-100 expression were positive, fluorescence signal located in the cytoplasm, while only 11 cases of HCC were positive, and there was a significant difference between both positive rates (100% vs 37. 9%, P<0. 01). In addition, with the degree of differentiation of HCC decreased, the expression of miR-100 showed a decreasing trend, positive rate of the well differentiation group and moderate differentiation group was significantly higher than the poor differentiation group ( P<0. 05 ) . Conclusion Detection of miR-100 expression in HCC tissue might be helpful to evaluate the pathologic grade of the tumors.