Genetic location of SCCmec-associated psm-mec in Staphylococcus hominis from blood culture
10.3969/j.issn.1673-4130.2015.01.011
- VernacularTitle:SCCmec 相关的 psm-mec 在血液来源人葡萄球菌中的基因定位
- Author:
Liang CHEN
;
Yongchang YANG
;
Daiwen XIAO
;
Hua YU
;
Hua LIU
;
Wenfang HUANG
- Publication Type:Journal Article
- Keywords:
SCCmec typing;
psm-mec gene;
Staphylococcus hominis
- From:
International Journal of Laboratory Medicine
2015;(1):27-29,32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic location of SCCmec-associated psm-mec in Staphylococcus hominis isolated from blood culture,and to lay a foundation for further functional studies of psm-mec in Staphylococcus hominis.Methods 25 strains of Staphylococcus hominis isolated from positive blood culture were collected.mecA and psm-mec gene were amplified by PCR,and the SCCmec types were determined by the results of multiplex PCR assay.For analyzing the genetic location characteristic of psm-mec in SCCmec,three pair special PCR primers were used to measure mecR1/psm-mec,psm-mec/xylR and fudoh respectively.Results There were 21 strains of methicillin-resistant Staphylococcus hominis and 4 strains of methicillin-sensitive Staphylococcus hominis. The positive rate of psm-mec gene in methicillin-resistant Staphylococcus hominis was 47.6%,and no psm-mec gene was found in methicillin-sensitive Staphylococcus hominis.Among psm-mec positive strains,2 strains belonged to SCCmecⅢ,5 strains belonged to SCCmecⅢ-like,and 3 strains belonged to new SCCmec types.All of the 10 psm-mec positive strains were mecR1/psm-mec,psm-mec/xylR and fudoh gene positive.Conclusion SCCmec-associated psm-mec extensively exists in methicillin-resistant Staphylococ-cus hominis isolated from positive blood culture,which distributes mainly in typical SCCmecⅢ,SCCmecⅢ-like and new SCCmec types and locates between mecR1 and xylR gene.
