Application analysis of double-disk synergy test in AmpC β-lactamase detection
10.3969/j.issn.1673-4130.2014.16.037
- VernacularTitle:双纸片抑制增效试验在 AmpC 酶检测中应用分析
- Author:
Gangsen ZHENG
;
Jiaqin ZHANG
;
Chaoyang HUANG
;
Xiaobo MA
- Publication Type:Journal Article
- Keywords:
double-disk synergy test;
Klebsiella.Pneumoniae;
AmpC;
Cefoxitin
- From:
International Journal of Laboratory Medicine
2014;(16):2208-2209,2211
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and analyze the double-disk inhibiting synergy test for detecting AmpC β-lactamase pro-duced by Klebsiella pneumoniae and to evaluate its application value in clinical laboratory.Methods The cefoxitin disk agar diffu-sion method,cefoxitin three-dimensional method,double-disk inhibiting synergy test and drug resistance gene multiplex PCR assay were adopted to detect the clinically isolated bacterial strains.Results Among 137 clinically isolated strains of Klebsiella pneumoni-ae,22 strains were insensitive to cefoxitin and 11 strains were positive by the three-dimensional method;in the double-disk inhibiting synergy test,18 strains were positive for the FOX/FOX+PBA group and 11 strains were positive for the CTT/CTT+PBA group respectively;in the multiplex PCR assay,19 strains were positive.The coincidence rate of the cefoxitin three-dimensional method and multiplex PCR methods was 47.4%(9/19),in the double-disk inhibiting synergy test,the coincidence rate of the positive re-sults in the CTT/CTT+PBA group and the multiplex PCR methods was 57.9%(11/19);the coincidence rate of the FOX/FOX+PBA group and multiplex PCR methods was 94.7%(18/19).Conclusion The double-disk inhibiting synergy test is simple with highly accurate results,in which the FOX/FOX+PBA double-disk synergy test could be applied to detect AmpC β-lactamase pro-duced by clinical isolates of Klebsiella pneumoniae.
