Protective effects of ghrelin against alcoholic induced liver injury in mice via anti-inflammation and anti-oxidative stress
10.3969/j.issn.1001-1978.2014.10.025
- VernacularTitle:脑肠肽通过抗炎抗氧化作用发挥酒精性肝损伤的保护作用
- Author:
Mengwen SUN
;
Shilian HU
;
Shi YIN
;
Guang YAN
- Publication Type:Journal Article
- Keywords:
ghrelin;
alcoholic liver injury;
oxygen free radicals;
TNF-α;
IL-1β;
MCP-1
- From:
Chinese Pharmacological Bulletin
2014;(10):1452-1455,1456
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of ghrelin on alcohol-induced liver injury. Methods The alcoholic liver injury mouse model was induced by chronic etha-nol feeding ( 4-week ad libitum oral feeding with the ethanol liquid diet) plus a single binge ethanol (5 g· kg-1 ) feeding. The level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) in serum, malondiadehyde ( MDA ) content, superoxide dis-mutase (SOD) and glutathione peroxidase (GSH-Px) activities in liver homogenate were assayed by spectro-photometer. Hepatic pathological examination was ob-served by HE staining. The mRNA expression of proin-flammatory cytokines including TNF-α, IL-1β, IFN-γ, IL-6 and MCP-1 in the liver was measured by real-time PCR method. Results This chronic-plus-single-binge high dose ethanol feeding synergistically induced liver injury, inflammation and fatty liver change. Treatment with Ghrelin ( 5 , 10 , 20 μg · kg-1 ) significantly de-creased the enhanced level of transaminase ( ALT, AST) in serum, improved the pathologic change in liv-er, and reduced the infiltration of inflammatory cells induced by alcohol administration. Ghrelin also de-creased MDA content and increased the reduced SOD and GSH-Px level in liver homogenate. Furthermore, ghrelin decreased inflammatory cytokines mRNA ex-pression including TNF-α, IL-1β, IFN-γ, IL-6 and MCP-1 in the liver. Conclusion Ghrelin has protec-tive effects against alcoholic liver injury in mice via in-hibiting inflammation and suppressing oxidative stress.
