Evaluation of quantitative detection limit and functional sensitivity of prealbumin and their clinical applications analysis
10.3969/j.issn.1673-4130.2014.14.039
- VernacularTitle:前清蛋白定量检测限和功能灵敏度的评价及临床应用
- Author:
Zemin WAN
;
Xiujuan ZHANG
;
Lei LI
;
Yunxiu WANG
;
Xiaobin WU
;
Weiye CHEN
;
Cheng ZHANG
;
Jianbing WANG
;
Peifeng KE
- Publication Type:Journal Article
- Keywords:
prealbumin;
immune nephelometry;
limit of blank;
limit of detection;
functional sensitivity
- From:
International Journal of Laboratory Medicine
2014;(14):1908-1909,1913
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the limit of blank (LoB),limit of detection (LoD),limit of quantitation(LoQ)and functional sensitivity (FS)of prealbumin (PA)detected by the Roche Modular P automatic biochemical analyzer.Methods According to the EP17A file of the American Clinical and Laboratory Standards Institute (CLSI),saline as the blank sample and a series of low con-centration samples were detected by the Roche Modular P automatic biochemical analyzer for determining LoB,LoD and LoQ.And FS was determined based on the domestic universal method .Results LoB of PA was 16.35 mg/L,LoD was 18.23 mg/L,LoQ was temporarily unable to evaluate and FS was 25.00 mg/L.The report scope and the report mode in clinic were affirmed by combi-ning with the low value of the reportable scope.Conclusion LoD of PA detected by the Roche Modular P automatic biochemical an-alyzer is established,which provides more valuable information for clinical diagnosis and treatment.Conducting the comparison of different evaluation methods determines the advantages and limitation of the practical application of different methods.
