Expression of monocyte-macrophage-related factors in kidney tissues of rats with ureter obstruction and recanalization
10.3969/j.issn.0529-1356.2014.03.020
- VernacularTitle:输尿管梗阻及再通大鼠肾组织中单核-巨噬细胞相关因子动态表达
- Author:
Hong LU
;
Rongrong YANG
;
Yong LIANG
;
Chengcheng LIN
;
Weilong HONG
;
Bicheng CHEN
;
Yongheng BAI
- Publication Type:Journal Article
- Keywords:
Monocyte-macrophage;
Chemoattractant factor;
Ureter obstruction;
Recanalization;
Fibrosis;
Immunohistochemistry;
Real-time PCR;
Rat
- From:
Acta Anatomica Sinica
2014;(3):405-410,420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of monocyte-macrophage-related factors and interstitial fibrosis in kidney tissues of rats with ureter obstruction and recanalization .Methods Forty-eight male Spragur-Dawley rats were divided randomly into the obstructive group:sham (n=6), unilateral ureteral obstruction(UUO)3 days (n=6), UUO 7 days (n=6), and UUO 14 days (n=6) and recanalization group:bilateral ureteral obstruction(RBUO)0 day (n=6), 3 days after RBUO (n=6), 7 days after RBUO (n=6), and 14 days after RBUO (n=6).The kidneys were excised on day 3, 7, and 14, and the deposition of collagen fibers in kidney was detected with HE and Masson staining . Immunohistochemical analysis was performed to evaluate the protein expressions of monocyte chemoattractant protein -1 (MCP-1), macrophage colony-stimulating factor (M-CSF) and activated-macrophage marker CD68.Real-time PCR was used to detect the mRNA expressions of MCP-1 and M-CSF.TGF-β1 levels were determined by ELISA .Results Fibrosis observed with HE and Masson staining was obviously increased in kidney tissue of UUO rats , and aggravated as time prolonged, but alleviated in rats with recanalization .TGF-β1 levels were increased obviously in the UUO group , but decreased in rats with recanalization compared with those in BUO rats .In UUO rats, mRNA and protein expression levels of MCP-1 and M-CSF were increased .MCP-1 and M-CSF expression was gradually decreased in rats with recanalization compared with those in BUO rats .The dynamic change in expression of MCP-1 and M-CSF in both UUO rats and recanalization rats was consistent with the change in expression of CD 68. Conclusion Dynamic change in expression of MCP-1 and M-CSF in kidney tissues reflects change of activated and accumulated monocyte -macrophages , which may be one of the major mechanisms contributing to fibrosis induced by ureter obstruction .Renal fibrosis is alleviated by down-regulated expression of monocyte-macrophages factors with recanalization operation .
