Experimental study on transfection of humanβ-NGF recombinant plasmid into BMSCs of GFP transgenic mouse
10.3969/j.issn.1671-8348.2014.10.003
- VernacularTitle:人β-NGF重组质粒转染GFP转基因小鼠BMSC的实验研究
- Author:
Qianghe LIU
;
Liangliang WANG
;
Xiang WANG
;
Yongbao WANG
;
Xin HUANG
;
Ming DENG
;
Fangxian LIU
- Publication Type:Journal Article
- Keywords:
nerve growth factor;
green fluorescent proteins;
lipofectamine 2000;
transfection;
mice;
bone marrow stromal stem cells
- From:
Chongqing Medicine
2014;(10):1161-1163,1167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological features of the mouse bone marrow stromal stem cells (BMSCs) transfected by humanβnerve growth factor(β-NGF) .Methods BMSCs of GFP transgenic mouse were isolated and cultured .Theβ-NGF re-combinant plasmid vectors were transferred into the cultured BMSCs by LipofectamineTM 2000 .The expression of β-NGF was detec-ted with ELISA .Results The β-NGF recombinant plasmid vectors were successfully transferred into BMSCs of GFP transgenic mouse ,the expression of β-NGF in transfected cells appeared .In addition ,the expression ofβ-NGF could effectively protect the BM-SCs which were injured in transfection process .Conclusion BMSCs of GFP transgenic mouse after transfecton with human β-NGF have the proliferation and differentiation capacity ,and possess the expression ability of β-NGF protein .
