Role of Toll-like receptor in the dendritic cell response during early Plasmodium yoelii infection
10.3969/j.issn.1002-2694.2009.07.004
- VernacularTitle:Toll样受体在约氏疟原虫感染早期树突状细胞应答中的作用地位
- Author:
Wei ZHENG
;
Jun LIU
;
Lu HAN
;
Hui FENG
;
Hongrui MENG
;
Yaming CAO
- Publication Type:Journal Article
- Keywords:
Plasmodium yoelii;
dendritic cell;
Toll like receptor
- From:
Chinese Journal of Zoonoses
2009;(7):627-629,635
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of Toll like receptor (TLR) in the activation of dendritic cells (DC) during early Plasmodium yoelii infection of the lethal strain 17XL (P.y 17XL), susceptible BALB/c and resistant DBA/2 mice were infected by i.p.injection of the P.y l7XL-parasitized erythrocytes, and the parasitemia of individua1 mice was monitored by the microscopic examination of blood smear stained with Giemsa.Mice from norma1 and infected groups were sacrificed on 0,3 and 5 days post-infection to collect their spleen cells.And the expressions of TLR-9 and TLR-4 on the cell surface of DCs in spenonocytes of these two strains of mice were assayed by applying flow cytometry to quantitatively analyze the percentages of CD11c+TLR9+ DCs and CD11c+TLR4+ DCs. It was found that the population of CD11c+DCs expressing TLR9 was significantly increased on day 3 and peaked on 5 p.i. in BALB/c (P<0.01) and DBA/2 mice(P<0.01). However, there was no statistical significance between these two strains of mice. Meanwhile, there was no change on the population of CD11c+ DCs expressing TLR4 in BALB/c and DBA/2 mice. These results indicate that TLR9 may contribute to the DC activation during early stages of P.y17XL infection.
