Effect of indomethacin on the migration of breast cancer cells in vitro
10.3760/cma.j.issn.1673-4181.2012.03.004
- VernacularTitle:吲哚美辛对乳腺癌细胞体外迁移能力的影响研究
- Author:
Dandan CHEN
;
Shaoguang YANG
;
Fengxia MA
;
Junjie CUI
;
Xue LI
;
Wenjing DU
;
Zhongchao HAN
- Publication Type:Journal Article
- Keywords:
Indomethacin;
Breast cancer;
Migration
- From:
International Journal of Biomedical Engineering
2012;35(3):142-145,后插3
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of indomethacin on the migration of breast cancer cell line MCF-7 in vitro and investigate the mechanism involved.MethodsThe migration of MCF-7 cell line stimulated with or without indomethacin were tested using transwell plates consisting upper and lower chambers separated by Millipore polycarbonate membrance filters with 8 μm pore sizes; the levels of chemokine receptor 4(CXCR4),cyclooxygenase(COX-2),epidermal growth factor receptor(EGFR) and vascular endothelial growth factor(VEGF)expression in MCF-7 cell line were detected by flow cytometry,Real-time PCR and ELISA,respectively.Results Indomethacin decreased the migration ability of MCF-7 cell line significandy.CXCR4 membrane expression was significantly reduced in a time-dose dependent manner,and CXCR4,COX-2 and EGFR mRNA levels were significantly downregulated after indomethacin stimulation.However,exposure to indometahcin had no major effect on VEGF production of cells.ConclusionThe downregulation of CXCR4,COX-2 and EGFR expression might be the primary mechanism involved in the inhibitory effect of indomethacin on the migration of MCF-7 cell line.
