Study on the cytotoxicity and gene transfection of NMPCS/DNA nanoparticles
10.3760/cma.j.issn.1673-4181.2012.03.009
- VernacularTitle:N-亚甲基磷酸化壳聚糖基因纳米粒子的体外细胞毒性及基因转染实验研究
- Author:
Liping SONG
;
Dunwan ZHU
;
Lanxia LIU
;
Xia DONG
;
Hai WANG
;
Jingen BAI
;
Xigang LENG
- Publication Type:Journal Article
- Keywords:
Methylene phosphonic chitosan;
Non-viral vector;
Gene transfection
- From:
International Journal of Biomedical Engineering
2012;35(3):165-168,后插7
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the cytotoxicity and gene transfection mediated by NMPCS/DNA nanoparticles.MethodsN-methylene phosphonic chitosan (NMPCS) was synthesized using one-step reaction under homogeneous conditions.The NMPCS/DNA nanoparticles were prepared using complex coacervation method.The cytotoxicity of NMPCS alone and its complexes with plasmid DNA were determined by MTT assay on HeLa cells.The gene transfection mediated by NMPCS/DNA nanoparticles were investigated using pGL3control vector as reporter gene.ResultsThe MTT results suggested that the NMPCS and NMPCS/DNA complexes showed significantly lower cytotoxicity than PEI and PEI/DNA complexes,respectively.The gene transfection mediated by NMPCS/DNA nanoparticles were greatly improved compared with unmodified chitosan.ConclusionNMPCS would demonstrate great potential as a novel,safe,efficient non-viral vector for gene delivery.
